In vitro pathogenicity of Metarhizium anisopliae strains in Rhicephalus (Boophilus) microplus
DOI:
https://doi.org/10.5377/ribcc.v7i13.11271Keywords:
Biocaricida, Ectoparasito, Cepa-CD0804, Boophilus, Metarhizium anisopliaeAbstract
Rhicephalus (Boophilus) microplus ticks represent a strong problem in cattle production systems in the Mexican humid tropics. For its control, various chemical products are applied with negative implications such as bio-resistance, high costs, environmental impact and risks to people's health due to the consumption of milk and meat with product residues. The objective of this research was to evaluate the in vitro pathogenicity of Metarhizium anisopliae strains MM0801 and CD0804 on Rhicephalus (Boophilus) microplus ticks. Five concentrations 3x104, 3x105, 3x106, 3x107, 2.6x108 of conidia per milliliter of M. anisopliae were evaluated on ticks in adult stage collected live from cattle from the Catazajá dairy basin, Chiapas, Mexico. Ticks were susceptible to the entomopathogenic fungus, finding a mortality of 50% where the mean lethal concentration obtained was 6.58 x 106 conidia / ml of strain MM0801. It is concluded that M. anisopliae influences the degree of virulence of the tick, which may be an alternative for the biological control of the tick in the humid Mexican tropics.
Downloads
Metrics
References
Baruch, Z. M. J. 2012. Prevalencia de ranchos y factores asociados con poblaciones de Rhipicephalus (Boophilus) microplus resistentes a cipermetrina en municipios de la zona centro del estado de Veracruz. Veracruz. Pag.10.
Bautista-Gálvez, A., Barrera, J.F., Payró de la Cruz, E., Salgado-García, S., Gómez-Ruiz, J., Gómez-Leyva, J.F. 2012. Genetic characterization of Metarhizium anisopliae (Metchnikoff) sorokin isolates from sugarcane field and their pathogenicity against Aeneolamia postica (Walker) (Hemiptera: Cercopidae). Universidad y Ciencia (28) (3):217-229. ISSN:0186-2979.
Bautista, G.A. Pimentel S.R. y Gómez V.A. 2017. Control ciológico de la garrapata Rhipicephalus (Boophilus) microplus con hongos entomopatógenos. Revista Iberoamericana de las Ciencias Biológicas y Agropecuarias. Vol. 6. Num. 12. Pag 1-29. ISSN: 2007-9990.
https://doi.org/10.23913/ciba.v6i12.68
Bazán, T. M. 2002. Efecto de Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) en el control biológico de Boophilus microplus Canestrini (Acari: Ixodidae) en ganado bovino estabulado. Tecomán, Colima. Pag. 9.
Elósegui, O., Nieves, C., Díaz, R., Bel, P.N., Carr, A. 2003. Comportamiento del hongo entomopatógeno Beauveria bassiana (Bals.) vuill. Cepa Lbb-1 en Agar Sabouraud Dextrosa producido en Cuba. Fitosanidad, Instituto de Investigación de Sanidad Vegetal. Cuba. Vol. 7(2). Pag. 49-53
García - Gutiérrez, C., Hernández - Velázquez, V. M., González - Maldonado, M. B. 2006. Hongos entomopatógeno. Biotecnología Financiera Aplicada a Bioplaguicidas. México D. F. pág. 100.
Goetell, S.M., Inglis, D.G. 1997. Fungi: Hyphomycetes. Manual of Techniques in Insect Pathology. Edic by: Lacey. A. L. Yakima Agricultural Research Laboratory. USDA. Pag. 213-244.
https://doi.org/10.1016/B978-012432555-5/50013-0
Lew-Tabor, A. E., Bruyeres, A. G., Zhang, B., Rodríguez, M. V. 2014. Rhipicephalus (Boophilus) microplus tick in vitro feeding methods for functional (dsRNA) and vaccine candidate (antibody) screening. Ticks and Tick-borne Diseases, 5, 500-510.
https://doi.org/10.1016/j.ttbdis.2014.03.005
Melo, D.R., Reis, R.C.S., Bittencourt, V.R.E.P. (2006) Patogenicidade In vitro do fungo Metarhizium anisopliae (Metschnikoff, 1879) Sorokin, 1883, sobre o garrapata Boophilus microplus (canastrini, 1887). Rev. Brass. Parasitol. Vet., 15, 4, 157-162.
Monroy, R., Lozano, E, Pimentel, R, Brindis, A, Hernández, F, Coutiño, R. 2016. Análisis Económico - Financiero de un Acopio Lechero en la Región Maya de Chiapas. KUXULKAB'- Tierra viva o naturaleza en voz Chontal-, 22(43), 45-54.
Ojeda-Chi, M.M., Rodríguez-Vivas, R.I., Galindo-Velasco, E., Lezama-Gutiérrez, R., Cruz-Vázquez, C. 2011. Control de Rhipicephalus microplus (Acari: Ixodidae) mediante el uso del hongo entomopatógeno Metarhizium anisopliae (Hypocreales: Clavicipitaceae). Revisión. Pag. 178, 182,183.
Ruiz-Sánchez, E., Chan-Cupul, W., Pérez, G.A., Cristóbal-Alejo, J., Uch-Vázquez, B., Tun-Suárez, J. M., Munguía-Rosales, R. 2011. Crecimiento, esporulación y germinación in vitro de cinco cepas de Metarhizium y su virulencia en huevos y ninfas de Bemisia tabaco. Revista Mexicana de Micología (33): 9-15. Pag. 12-13.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Ibero-american JournalL of Bioeconomy and Climate Change e-ISSN 2410-7980
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Copyright © 2024 Rev. iberoam. bioecon. climate change. National Autonomous University of Nicaragua León (UNAN-León), Area of Knowledge of Agricultural and Veterinary Sciences / Specific Area of Agroecology / Center for Research in Bioeconomy and Climate Change (CIByCC)..