Antioxidant Capacity and Phenolic Compound Content Present in the Pulp of Psidium guajava L. and Persea americana Mill.

Authors

  • S. N. Peralta-Vanegas Specific Directorate of Biology, Area of ​​Knowledge of Science and Technology, National Autonomous University of Nicaragua, León, Nicaragua. https://orcid.org/0009-0005-2714-5183
  • J. J. Aviles-Betanco Specific Directorate of Biology, Area of ​​Knowledge of Science and Technology, National Autonomous University of Nicaragua, León, Nicaragua. https://orcid.org/0009-0007-1625-8670
  • A. S. Delgado-González Specific Directorate of Biology, Area of ​​Knowledge of Science and Technology, National Autonomous University of Nicaragua, León, Nicaragua. https://orcid.org/0009-0008-4301-6818
  • S. J. Flores-Blanco Specific Directorate of Biology, Area of ​​Knowledge of Science and Technology, National Autonomous University of Nicaragua, León, Nicaragua. https://orcid.org/0009-0009-3456-4529
  • M. E. Cerda-Castillo Specific Directorate of Biology, Area of ​​Knowledge of Science and Technology, National Autonomous University of Nicaragua, León, Nicaragua. https://orcid.org/0009-0005-8727-7014
  • C. M. Dolmus-Bustillo Specific Directorate of Biology, Area of ​​Knowledge of Science and Technology, National Autonomous University of Nicaragua, León, Nicaragua. https://orcid.org/0000-0002-7280-939X

DOI:

https://doi.org/10.5377/ribcc.v12i23.23848

Keywords:

Aguacate, capacidad antioxidante, guayaba, fenoles totales

Abstract

Background: Although Psidium guajava L. and Persea americana Mill. are widely consumed and recognized for their nutritional value, local scientific evidence concerning their antioxidant profiles determined under standardized analytical methods remains scarce. This knowledge gap limits their potential exploitation in health-related and economic applications. This study aimed to evaluate the total phenolic content and antioxidant capacity in the pulp of Psidium guajava L. (guava) 
and  Persea americana Mill. (avocado). Methodology: Thirty grams of pulp were analyzed using three biological replicates. The treatments consisted of evaluating 80 % ethanolic extracts obtained from the pulps of both fruits. Spectrophotometric 
methods were applied, including Folin-Ciocalteu for total phenolic content (TPC),  alongside DPPH and FRAP assays for antioxidant capacity. Results: Psidium guajava L. exhibited a higher antioxidant capacity in the DPPH assay (65.40%) and a total  phenolic content of 52.08%, attributable to its high concentration of vitamin C and water-soluble compounds. Conversely, Persea americana Mill. showed superiority in the FRAP assay (64.83%) and a total phenolic content of 47.93%, standing out for its significant reducing-power antioxidants. Conclusion: Both fruits exhibit complementary antioxidant profiles; P. guajava is rich in hydrophilic compounds, while P. americana excels in lipophilic content, demonstrating high potential for the development of functional foods.

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Author Biographies

S. J. Flores-Blanco, Specific Directorate of Biology, Area of ​​Knowledge of Science and Technology, National Autonomous University of Nicaragua, León, Nicaragua.

Research professor at the Specific Directorate of Biology, Area of ​​Knowledge of Science and Technology, National Autonomous University of Nicaragua, León, Nicaragua.

M. E. Cerda-Castillo, Specific Directorate of Biology, Area of ​​Knowledge of Science and Technology, National Autonomous University of Nicaragua, León, Nicaragua.

Research professor at the Specific Directorate of Biology, Area of ​​Knowledge of Science and Technology, National Autonomous University of Nicaragua, León, Nicaragua.

C. M. Dolmus-Bustillo, Specific Directorate of Biology, Area of ​​Knowledge of Science and Technology, National Autonomous University of Nicaragua, León, Nicaragua.

Research professor at the Specific Directorate of Biology, Area of ​​Knowledge of Science and Technology, National Autonomous University of Nicaragua, León, Nicaragua.

References

Bhuyan, D. J., Alsherbiny, M. A., Perera, S., Low, M., Basu, A., Devi, O. A., Barooah, M. S., Li, C. G., & Papoutsis, K. (2019). The Odyssey of Bioactive Compounds in Avocado (Persea americana) and Their Health Benefits. Antioxidants, 8(10), article 426. https://doi.org/10.3390/antiox8100426

Brand-Williams, M. C. (1995). Use of a free radical method to evaluate antioxidant activity. Food Science and Technology, 28(1), 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5.

Bravo Sánchez L. R. & Quihuiri Quiguiri P. H. (2021). Revisión acerca del potencial antioxidante de las frutas amazónicas: Eugenia stipitata mc vaugh (arazá) y psidium guajava L. (guayaba) y su aprovechamiento agroindustrial [Universidad Estatal Amazónica]. https://repositorio.uea.edu.ec/handle/123456789/1364

Carranza-Téllez, J., Torres-Hernández, D. M., Contreras-Martínez, C. S., García González, J. M., & Carranza-Concha, J. (2024). Influencia en la capacidad antioxidante de los fenoles totales, vitamina C y color en frutas. Revista Fitotecnia Mexicana, 47(1), 19–26. https://doi.org/10.35196/rfm.2024.1.19

Choi, B., & Shin, Y. (2025). Comparative Analysis of Physicochemical Characteristics, Antioxidant Compound Contents, and Antioxidant Activities of Five Guava (Psidium guajava L.) Cultivars Harvested in Korea. Foods, 14(21), 3645.https://doi.org/10.3390/foods14213645

Das, B.., Hussain, M.., Lalrinthari, Phukan, T.. y Mossadique, S.. (2026). Actividad antioxidante in vitro del extracto de hoja de Psidium guajava L. Revista Internacional de Ciencias Farmacéuticas y Biomédicas, 6 (04), 965–971. https://doi.org/10.47191/ijpbms/v6-i4-07

Dibacto, R. E. K., Tchuente, B. R. T., Nguedjo, M. W., Tientcheu, Y. M. T., Nyobe, E. C., Edoun, F. L. E., Kamini, M. F. G., Dibanda, R. F., & Medoua, G. N. (2021). Total Polyphenol and Flavonoid Content and Antioxidant Capacity of Some Varieties of Persea americana Peels Consumed in Cameroon. The Scientific World Journal, article, 8882594. https://doi.org/10.1155/2021/8882594

Dreher, ML, y Davenport, AJ (2013). Composición del aguacate Hass y posibles efectos sobre la salud. Revisiones críticas en ciencia de los alimentos y nutrición, 53 (7), 738-750. https://doi.org/10.1080/10408398.2011.556759

Gobierno de Reconciliación y Unidad Nacional [GRUN]. (2021). Plan Nacional de Lucha contra la Pobreza y para el Desarrollo Humano 2022-2026. Managua, Nicaragua.

Gutierrez Montiel, D., Guerrero Barrera, A. L., Martínez Ávila, G. C. G., Gonzalez Hernandez, M. D., Chavez Vela, N. A., Avelar Gonzalez, F. J., & Ramírez Castillo, F. Y. (2024). Influence of the Extraction Method on the Polyphenolic Profile and the Antioxidant Activity of Psidium guajava L. Leaf Extracts. Molecules, 29(1), article 85. https://doi.org/10.3390/molecules29010085

Thaipong, K., Boonprakob, U., Crosby, K., Cisneros-Zevallos, L., & Byrne, D. H.(2006). Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. The Journal of Food Composition and Analysis, 19 (6-7) 669-675. https://doi.org/10.1016/j.jfca.2006.01.003

Kumar, M., Tomar, M., Amarowicz, R., Saurabh, V., Nair, M. S., Maheshwari, C., Sasi, M., Prajapati, U., Hasan, M., Singh, S., Changan, S., Prajapat, R. K., Berwal, M. K., & Satankar, V. (2021). Guava (Psidium guajava L.) Leaves: Nutritional Composition, Phytochemical Profile, and Health-Promoting Bioactivities. Foods, 10(4), 752. https://doi.org/10.3390/foods10040752

Lakshmi, M., P, S. S., & K, S. (2022). Guava (Psidium guajava L.): A brief overview of its therapeutic and health potential. Journal of Pharmaceutical Negative Results, 13(S1), 470–475. https://doi.org/10.47750/pnr.2022.13.S01.059

Lyu, X., Agar, O. T., Barrow, C. J., Dunshea, F. R., & Suleria, H. A. R. (2023). Phenolic Compounds Profiling and Their Antioxidant Capacity in the Peel, Pulp, and Seed of Australian Grown Avocado. Antioxidants (Basel, Switzerland), 12(1), article 185. https://doi.org/10.3390/antiox12010185

Mendoza Carpio, V. E. (2024). Evaluación de la actividad antioxidante de subproductos del aguacate (Persea americana Mill. variedad Hass) [Trabajo de titulación, Universidad de Cuenca]. Repositorio Institucional de la Universidad de Cuenca. https://dspace.ucuenca.edu.ec/items/61289b1e-0dce-406b-900afebdf43e74a7

Petrantonaki, A., Theodoropoulou, V.-I., Mantiniotou, M., Athanasiadis, V., Bozinou, E., & Lalas, S. I. (2025). Optimization of Polyphenol-Rich Extracts from Defatted Avocado Peel and Seed Residues Using Ultrasound-Assisted RSM: Antioxidant Potential and Valorization Prospects. Antioxidants, 14(11), article 1293. https://doi.org/10.3390/antiox14111293

Rojas-Barquera, D., & Narváez-Cuenca, C. E. (2009). Determinación de vitamina C, compuestos fenólicos totales y actividad antioxidante de frutas de guayaba (Psidium guajava L.) cultivadas en Colombia. Química Nova, 32(9), 2336–2340. https://doi.org/10.1590/S0100-40422009000900019

Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods in Enzymology, 299, 152–178. https://doi.org/10.1016/S0076-6879(99)99017-1

Skoog, D. A., Holler, F. J., & Crouch, S. R. (2017). Principles of Instrumental Analysis (7th ed.). Cengage Learning.

Xin, Z., Gao, Y., He, L., Xiu, Z., & Sun, L. (2025). Bioactive Antioxidants from Avocado By-Products: Mechanistic Study and Laboratory-Scale Extraction Optimization. Antioxidants, 14(10), article 1225. https://doi.org/10.3390/antiox14101225

Yadav, A., Kumari, R., Yadav, A., Mishra, J. P., Srivatva, S., & Prabha, S. (2020). Antioxidant Activity of Psidium guajava Leaf Extracts. Research Journal of Pharmaceutical Dosage Forms and Technology, 9(5), 140–144. https://doi.org/10.5958/0975-4377.2020.00027.0

Zar, J. H. (2010). Biostatistical Analysis (5th ed.). Pearson Education.

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Published

2026-10-16

How to Cite

Peralta-Vanegas, S. N. . . . ., Aviles-Betanco, J. J. . . ., Delgado-González, A. S., Flores-Blanco, S. J., Cerda-Castillo, M. E., & Dolmus-Bustillo, C. M. (2026). Antioxidant Capacity and Phenolic Compound Content Present in the Pulp of Psidium guajava L. and Persea americana Mill. Ibero-American Journal of Bioeconomics and Climate Change, 12(23), 2624–2628. https://doi.org/10.5377/ribcc.v12i23.23848

Issue

Vol. 12 No. 23 (2026): Special Edition: Winners XLIII JUDC 2025

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Research articles

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Copyright (c) 2026 Ibero-American Journal of Bioeconomics and Climate Change

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Copyright © 2025 Rev. iberoam. bioecon. climate change. National Autonomous University of Nicaragua León (UNAN-León), ​​Knowledge Area of Agrarian and Veterinary Sciences / Specific Area of ​​Agroecology and Agribusiness / Center for Research in Agrarian Sciencies. Academic Directorate. Research Department. Publication and scientific events Unit. 

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