Trypanosomiasis is a debilitating and commonly fatal disease in domestic animals, especially in cattle and small ruminants. It is caused by several species of Trypanosomes, mainly Trypanosoma vivax. The aim of this study is to describe the prevalence of Trypanosoma in symptomatic ovines on a farm in the municipality of León, Nicaragua. A total of 100 pelibuey ovines were analyzed with symptomatology with the giemsa staining technique and Indirect Immunofluorescence (IFI). The presence of Trypanosomes was identified in 47% of the Giemsa stain and 85% of the anti-trypanosomes antibodies by the IFI technique. The females were more affected than the males. The most relevant clinical characteristic presented by the pelibuey was weakness. It is recommended to carry out randomized studies in different areas and times of the year in order to establish endemicity curves.